![]() ![]() ![]() Neuroblastoma is the second most common solid tumor diagnosed during infancy. Zhan, Qinglei Tsai, Sauna Lu, Yonghai Wang, Chunmei Kwan, Yiuwa Ngai, Saiming RuvBL2 Is Involved in Histone Deacetylase Inhibitor PCI-24781-Induced Cell Death in SK-N-DZ Neuroblastoma Cells Our data suggest that SK-N-BE(2C cells have potential utility to serve as another model system to detect norepinephrine reuptake inhibition activity. The results obtained with the SK-N-BE(2C cells indicate that this model system can detect a range of ligand potencies, which compare well with other established transporter assays. Using this determined KM value, reuptake inhibition assays were then conducted with a variety of ligands including antidepressants, as well as piperazine and phenyltropane derivatives. Before conducting the assays, the SK-N-BE(2C cells were first evaluated for their ability to uptake norepinephrine and were shown to have a saturable uptake with a KM value of 416 nM. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer's disease.ĭevelopment of norepinephrine transporter reuptake inhibition assays using SK-N-BE(2C cellsĭirectory of Open Access Journals (Sweden)įull Text Available This report describes efforts to develop and validate novel norepinephrine transporter reuptake inhibition assays using human neuroblastoma SK-N-BE(2C cells in 24-well format. Phosphorylation of IÎ✫ and IKK was significantly (p pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. PWE (25-200 μg/ml) dose dependently reduced COX-2-dependent PGE2 production in SK-N-SH cells stimulated with IL-1β. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA. Expression of COX-2, phospho-IÎ✫, and phospho-IKK proteins was evaluated, while NF-Î✫ reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of PWE on NF-Î✫ transactivation. An enzyme immunoassay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1β-stimulated SK-N-SH cells. In this study, we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE2 production in IL-1β-stimulated SK-N-SH cells. (Punicaceae), and its constituents have been shown to inhibit inflammation. Velagapudi, Ravikanth Baco, Gina Khela, Sunjeet Okorji, Uchechukwu Olajide, Olumayokun Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells. ![]()
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